Endo Agar

<body topmargin=0 leftmargin=0 marginheight=0 marginwidth=0> <table cellpadding=0 cellspacing=0 border=0><tr> <td valign="top" colspan=2 height=90 BGCOLOR="#CCFF99" TEXT="#080000" bgproperties="fixed" background="04078671esnimw.png"> <div> <FONT SIZE="5" COLOR="#CCFF99" FACE="Arial" ><B>AVIANITARIAN Reference Guide </B></FONT><B>     |     </B><A HREF="index_avianitarian.htm" TARGET="_top" TITLE="AVIANITARIAN Reference Guide "><B>home</B></A></div> <div> <A HREF="id150.htm" TARGET="_top" TITLE="Lab Safety Practices">Lab Safety Practices</A>   |   <A HREF="id177.htm" TARGET="_top" TITLE="Lab Station">Lab Station</A>   |   <A HREF="id146.htm" TARGET="_top" TITLE="Lab Cleaning Responsibility">Lab Cleaning Responsibility</A>   |   <A HREF="id122.htm" TARGET="_top" TITLE="Washing Hands">Washing Hands</A>   |   <A HREF="id163.htm" TARGET="_top" TITLE="Aseptic Technique and Transfer of Microo">Aseptic Technique and Transfer of Micro-organisms</A>   |   <A HREF="id164.htm" TARGET="_top" TITLE="Enterotube">Enterotube</A>   |   <A HREF="id108.htm" TARGET="_top" TITLE="bacteria agars">bacteria agars</A>   |   <A HREF="id109.htm" TARGET="_top" TITLE="Biggy Agar">Biggy Agar</A>   |   <A HREF="id152.htm" TARGET="_top" TITLE="Isolation Pseudomonas/entre">Isolation Pseudomonas/entre</A>   |   <A HREF="id110.htm" TARGET="_top" TITLE="Sabouraud Destrose Agar">Sabouraud Destrose Agar</A>   |   <A HREF="id115.htm" TARGET="_top" TITLE="MacConkey">MacConkey</A>   |   <A HREF="id116.htm" TARGET="_top" TITLE="CLED Agar">CLED Agar</A>   |   <A HREF="id117.htm" TARGET="_top" TITLE="BORDET GENGOU AGAR BASE">BORDET GENGOU AGAR BASE</A>   |   <A HREF="id176.htm" TARGET="_top" TITLE="Specimen Collection">Specimen Collection</A>   |   <B>Endo Agar</B></div> <div> </div> </td> </tr><tr> <td valign="top" width=200 BGCOLOR="#336699" TEXT="#080000" bgproperties="fixed" background="041c83b3.png"> <bR> </td> <td valign="top" height=390 width="100%" BGCOLOR="#CCFF99" TEXT="#080000"> <div> <I>Endo Agar</I></div> <bR> <bR> <div> INTENDED USE.  Endo Agar is a differential and slightly selective culture medium for the detection of coliform and other enteric microorganisms</div> <bR> <div> SUMMARY AND EXPLANATION OF THE TEST.  The majority of the enteric plating media developed in the early years of the 20th century utilized ether mixtures of bile salts or individual salts as selective agents to achieve inhibition of gram-positive species. In 1904, Endo reported the development of a culture medium for the differentiation of lactose ferrnenters from the nonfermenters in which no bile salts were used. Inhibition of gram-positive microorganisms was achieved by the sodium sulfite and basic fuchsin contained in the formulation Endo's Fuchsin Sulphite Infusion Agar was the original name for this medium,  which is known today as Endo Agar. It was developed initially in order to facilitate the isolation and identification of the typhoid bacillus.</div> <bR> <div> The original formula has been modified extensively since its introduction. The meat infusions have been replaced by a peptic digest of animal tissue The dye composition and concentration also have been adjusted. </div> <bR> <div> Over the years, Endo Agar has been an important medium in the microbiological examination of potable water and wastewater, dairy products and foods, however, the current compendia of standard methods for the examination of these materials recommend alternative media formulations.</div> <bR> <div> PRINCIPLES OF THE PROCEDURE. The selectivity of Endo Agar is due to the sodium sulfite/basic fuchsin combination which results in the suppression of gram-positive microorganisms. It is classified as only slightly selective since other media contain more potent inhibitors of the gram-positive microorganisms. Coliforms ferment the lactose, producing pink to rose-red colonies and similar coloration of the medium. The colonies of organisms that do not ferment lactose are colorless to faint against the pink background of the medium.</div> <bR> <div> CLASSICAL FORMULA* PER LITER PURIFIED WATER</div> <div> Dipotassium Phosphate 3 5 g</div> <div> Peptic Digest of Animal Tissue 10 0</div> <div> Agar 150</div> <div> Lactose 10 0</div> <div> Sodium Sulfite 2.5</div> <div> Basic Fuchsin 0 5</div> <div> *Adjusted and/or supplemented as reduced to meet performance criteria Final pH 7 4 ± 0 2</div> <bR> <div> DIRECTIONS FOR PREPARATION FROM DEHYDRATED PRODUCT</div> <div> 1 Suspend 415 g of the Powder in 1 L of Purified Water.  Mix thoroughly.</div> <div> 2 Heat with frequent agitation and boil for 1 mm to completely dissolve the powder.</div> <div> 3 Sterilize by autoclaving at 12VC for 15 mm.</div> <div> 4 The medium should be cooled to approximately 450 C and the precipitate resuspended by gentle mixing before use. It is recommended that Endo Agar be prepared as needed.</div> <div> 3 Test samples of the finished product for performance using stable, typical control cultures.</div> <bR> <div> USER QUALITY CONTROL</div> <div> Escherichia coli Colonies pink to rose-red with green metallic sheen</div> <div> Marked reddening of the medium may occur</div> <div> ATCC~ 25922 </div> <div> Salmonella typhimurium Colonies colorless to faint pink</div> <div> ATCC 14028</div> <div> Streptococcus faecalis Inhibited Moderate growth acceptable</div> <div> Colonies small, pink to rose-red  Trace sheen may be evident</div> <div> ATCC 29212 </div> <bR> <div> PROCEDURE. Streak the specimen as soon as possible after it is received in the laboratory. The streak plate is used primarily to isolate pure cultures from specimens containing mixed flora. A nonselective medium should also be streaked to increase the chance of recovery when the population of gram-negative organisms is low and to provide an indication of other organisms present in the specimen. Alternatively, if material is being cultured directly from a swab, roll the swab over a small area of the surface at the edge, then streak from this inoculated area. Incubate plates, protected from light, at 35 ± 2'C for 18 to 24 h. If negative after 24 h, reincubate an additional 24 h.</div> <bR> <div> EXPECTED RESULTS. After incubation most plates will show an area of confluent growth. Because the streaking procedure is, in effect, a "dilution" technique, diminishing numbers of microorganisms are deposited on the streaked areas. Consequently, one or more of these areas should exhibit isolated colonies of the organisms contained in the specimen. Better isolation is obtained due to the inhibitory action of the medium.</div> <bR> <div> Typical colonial morphology on Endo Agar is as follows</div> <div> Escherichia coli Pink to rose-red, green metallic sheen</div> <div> Enterobacter/Klebsiella Large, mucoid, pink</div> <div> Proteus Colorless to pale pink</div> <div> Salmonella Colorless to pale pink</div> <div> Shigella Colorless to pale pink</div> <div> Pseudomonas Irregular, colorless</div> <div> Gram-positive bacteria No growth to slight growth</div> <bR> <bR> <bR> <div> REFERENCES</div> <div> I Endo 1904 Zentralbl Bakteriol, Abt 1, Orig 35109</div> <div> 2 Levin and Schoenlein 1930 A compilation of culture media for the cultivation of microorganisms Wiiiian,s & Wilkins, Baltimore</div> <div> 3 Greenberg, Trussell and Clesceri (ed ) 1985 Standard methods for the examination of water and wastewater 1 6th ed APHA, Washington, D C</div> <div> 4 Richardson (ed) 1985 Standard methods for the examination of dairy products, 1 ~th ed APHA, Washington, D C</div> <div> 5 Speck ted) 1984 Compendium of methods for the microbiological examination of foods, 2nd ed APHA, Washington, D C</div> <div> BBL® CATALOG NO. ORDERING INFORMATION:</div> <div> Endo Agar</div> <div> Cat No 11199 Dehydrated-500g</div> <div> 21167 Prepared Plates-Pkg of 20</div> <div> 21265 Prepared Plates-Ctn of 100</div> <bR> <bR> </td> </tr></table></body>